Analysis of SOD Gene Expression in Alicyclobacillus acidoterrestris under Heat and Acid Stresses
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Abstract:
Based on the complete sequence of the SOD gene cloned from Alicyclobacillus (A). acidoterrestris DSM 3922, quantitative real time polymerase chain reaction (PCR) was used to analyze the differences in SOD mRNA expression levels in A. acidoterrestris under acid and heat stresses. The results showed that the SOD gene of A. acidoterrestris was constitutively expressed under normal growth conditions and was quickly upregulated under acid and heat stress conditions. SOD expression level after 0.5 h of acid stress was 4.63 times higher than that of the control group and then increased to a maximum level, which was 32.55 times higher than that of the control group after one hour of acid stress. Subsequently, the SOD gene expression level rapidly decreased and was 1.67 times higher than that of the control group when acid stress was applied for five hours. When heat stress was applied at 70 ℃ for five minutes, the relative expression level of the SOD gene was 2.81 times higher than that of the control group and then increased to a maximum level, which was 15.05 times higher than that of the control after 25 minutes of heat stress. Subsequently, the SOD gene expression level rapidly decreased and was 4.93 times higher than that of control group when heat stress was applied for 40 minutes. A rapid response is a characteristic of SOD gene expression in A. acidoterrestris under heat and acid stresses, indicating that the SOD gene of A. acidoterrestris was closely related to the unique physiological mechanism for thermoacidophilic adaptation of A. acidoterrestris.
