Preliminary Studies on the Hypolipidemic Effect and Mechanism of Cyclocarya paliurus Polysaccharides in Hyperlipidemic Mice
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Abstract:
To preliminarily investigate the hypolipidemic mechanism and effect of Cyclocarya paliurus polysaccharides (CPP) in hyperlipidemic mice, the mice were randomly divided into model control, blank control, simvastatin, low dose CPP, medium dose CPP, and high dose CPP groups (16 mice per group). Model control and blankcontrol groups were orally administered with equivoluminal bacteria-free water. The simvastatin group was administered with a suspension of 4 mg/kg simvastatin and carboxymethylcellulose sodium (CMC-Na; 0.5%). The low-dose, medium-dose, and high-dose CPP groups were administered with 100 mg/kg, 200 mg/kg, and 400 mg/kg CPP water solutions, respectively. After four weeks of feeding, the lipopolysaccharide (LPS) activity and the levels of total cholesterol (TC), triglycerides (TG), high-density lipoprotein-cholesterol (HDL-C), low-density lipoprotein-cholesterol (LDL-C), HDL-C, and free fatty acids (FFA) were determined. The liver was removed to assess the pathological morphology and reverse transcription polymerase chain reaction (RT-PCR) was employed to detect the expression level of hormone-sensitive lipase (Hsl) mRNA. The results indicated that CPP induced a significant reduction in TC, TG, and LDL-C levels and LPS activity (P < 0.01), a significant increase in HDL-C and FFA levels (P < 0.01), and different degrees of improvement in the degree of hepatic steatosis when compared to the model group. Compared with the model group, a remarkable increase of Hsl mRNA expression was observed in CPP groups (P < 0.05). In summary, CPP can significantly decrease blood lipid levels and improve hepatic steatosis due to excessive uptake of lipids in hyperlipidemic mice.
