Protective Effect of Mulberry Polysaccharides on H2O2-induced Oxidative Damage in PC-12 Cells
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Abstract:
Mulberry polysaccharide T2 was obtained by water extraction and ethanol precipitation. Proteins were removed by salting out with 4% (mass fraction) ZnSO4 at a rate of 88.52%, to yield mulberry polysaccharide T3, which was isolated and purified by DEAE-52 cellulose ion exchange chromatography. Thus, four fractions: T3-1, T3-2, T3-3, and T3-4 were collected. T3-1 and T3-4 were the main components of mulberry polysaccharide T3. Subsequently, the antioxidant activity of the four components was measured in terms of protective effect on cellular oxidative damage. The cellular oxidative damage model was established after damage of PC-12 cells was induced by treatment with 700 μmol/L H2O2 for eight hours. Effects of all T3 fractions on normal PC-12 cellular proliferation were investigated and the results showed that T3-3 improved cell survival rate to 41.81%. Finally, the protective effect of T3-3 on H2O2-induced oxidative damage in PC-12 cells was investigated. The results showed that 600 μg/mL T3-3 exhibited the strongest protective effect, indicating that T3-3 exerts very strong cellular antioxidant activity at high concentrations.
