Effect of Unsaturated Fatty Acids from Rice Bran Oil on Cloning and Migration Ability of HepG2 Cells
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Abstract:
The in vitro effects of unsaturated fatty acids of rice bran oil (RBO-UFA) on HepG2 cells were examined by soft agar colony formation and transwell migration assays. Changes in the expression of the apoptosis-related factor, NF-κB, before and after RBO-UFA treatment were analyzed by western blot assay. Hoechst staining indicated that the number of HepG2 cells after RBO-UFA treatment was significantly decreased and the cytoplasm and nuclear chromatin were condensed. The soft agar colony formation assay showed that the HepG2 colonies were markedly reduced after RBO-UFA treatment and no colonies appeared. The migration assay indicated that the inhibition rates of linolenic acid and linoleic acid on the migration ability of HepG2 cells were up to 47.45 ± 4.60% and 41.61±4.08%, respectively, followed by that of rice bran oil (32.85 ± 3.24%) and that of oleic acid (25.55 ± 2.56%) (P < 0.05). The western blot results showed that NF-κB expression levels in total proteins from RBO-UFA treated groups were significantly lower than that of the normal control group. Compared with the control group, the relative expression levels of NF-κB in HepG2 cells treated with rice bran oil, oleic acid, linoleic acid, and linolenic acid were reduced by 0.46 ± 0.017, 0.058 ± 0.018, 0.25 ± 0.005, and 0.47 ± 0.011, respectively. On the other hand, compared with the control group, NF-κB expression in the RBO-UFA treated groups in the nuclear protein fraction was higher than that in the normal control group. The relative expression levels of NF-κB in HepG2 cells treated with rice bran oil, oleic acid, linoleic acid and linolenic acid were increased by 0.59 ± 0.036, 0.053 ± 0.040, 0.28 ± 0.043, and 0.59 ± 0.007 (P < 0.05), respectively. In summary, RBO-UPA treatment presented an inhibitory effect on the migration and clonogenicity of HepG2 hepatic cancer cells.
