Simultaneous Determination of Six Aflatoxins in Food by Immunoaffinity Purification-high Performance Liquid Chromatography Combined with a Large Volume Flow Cell
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
A method without derivative based on immunoaffinity purification and fluorimetric detection combined with large volume flow cell was developed for simultaneous determination of six kinds of aflatoxins in food. The samples were ultrasonic-extracted with acetonitrile-aqueous solution (84:16, V/V), cleaned up by immunoaffinity column, and then were separated by XBridgeTM C18 column (150 mm×4.6 mm, 5μm). The analytes were detected by large volume flow cell fluorescence detection in a mobile phase of acetonitrile-aqueous solution (84:16, V/V) without derivatization. External standard method was used for quantitative. All analytes were successfully separated within 6 min, and one sample determination from pretreatment to result analysis was finished within 50 min. The detection limit (LOD, S/N=3) of aflatoxin B1, B2, G1, G2, M1, M2 respectively were 0.05 μg/kg, 0.02 μg/kg, 0.05 μg/kg, 0.02 μg/kg, 0.04 μg/kg, 0.03 μg/kg, meeting the national limits of aflatoxins in food. The correlation coefficient of six kinds of aflatoxins, r2, were more than 0.999, and the spiked recoveries were in the ranges of 77.6%~90.5%, and relative standard deviation (RSD, n=3) were 2.42%~6.08%. The method was simple, effective and sensitive, detecting the aflatoxins without derivatization and suitable for the simultaneous determination of six kinds of aflatoxins in food.
