Structure and Thermal Properties of Glutelin from Euryale ferox Seeds
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
The Osborne procedure was used to extract glutelin from Euryale ferox, and the subunit molecular weight, thermal properties, and secondary structures of glutelin were preliminarily studied using reducing and non-reducing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), differential scanning calorimetry (DSC), and Fourier transform infrared spectroscopy (FT-IR), respectively. The results showed that E. ferox glutelin mainly contained six subunits, and most of the high-molecular-weight polypeptide chains were connected through intermolecular or intramolecular disulfide bonds. The highest denaturation enthalpy(ΔH) and temperature (Td) of E. ferox glutelin were observed at the isoelectric pH (pH 5.0), while the lowest ΔH and Td were observed at an alkaline pH (pH 9.0). The progressive increase in the Td of E. ferox glutelin and no pronounced changes in the ΔH were observed with increasing salt concentrations, suggesting salt-induced formation of of a conformation with higher thermal stability for E. ferox glutelin. FTIR analysis indicated that the main structure of E. ferox glutelin was ordered, and the proportion of β-sheet (31.16%) was the highest in the secondary structures. The present results indicate that E. ferox glutelin has numerous disulfide bonds, good thermal stability, and a stable structure. These results provide a theoretical basis for future studies of the functional characteristics and application of E. ferox glutelin.
