Study of the Separation, Purification, and Anti-tumor Activities of Peptides Prepared from Enzymolyzed Cuora trifasciata Meat
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
Here, minced Cuora trifasciata meat was used as a raw material from which peptides with anti-tumor activities were prepared by controlled enzymolysis. Alcalase, papain, trypsin, and protamex were used to carry out the hydrolysis of minced Cuora trifasciata meat, and 12 different molecular weight hydrolyzates were obtained after ultrafiltration. A 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay showed that hydrolyzate E, produced from tryptic digestion with a molecular weight of <3000 u, exhibited the best anti-tumor effect. When the concentration was 1 mg/mL, the anti-proliferation rates of hydrolyzate E against human liver cancer cells (HepG-2) and human breast cancer cells (MCF-7) were 92.95% and 67.08%, rpesectively. Hydrolyzate E was further purified by Sephadex G-15 gel filtration, and two polypeptide fractions (E1 and E2) with strong activity were selected based on an MTT assay. E1 had a stronger anti-proliferative effect on HepG-2 cells (IC50 = 77.29 μg/mL) than the positive control 5-fluorouracil (IC50 = 136.27 μg/mL), while E2 had a stronger anti-proliferative effect on MCF-7 cells (IC50 = 214.17 μg/mL) than the positive control 5-fluorouracil (IC50 = 245.82 μg/mL). In this study, peptides with anti-tumor activity were isolated from Cuora trifasciata for the first time, which will have important implications in the development of anti-tumor drugs and health products using Cuora trifasciata as the raw material.
