Molecular Typing of Salmonella Species Isolated from Beef in a Processing Plant with Fluorescence Amplified Fragment Length Polymorphism
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Abstract:
Fluorescence amplified fragment length polymorphism (FAFLP) analysis was used for molecular typing of 83 Salmonella strains in seven different serotypes isolated from seven different processing points, in order to investigate the contamination of Salmonella before and during slaughtering, to specify the main steps involving the contamination of Salmonella within the processing plant, and to provide data for the control of Salmonella infection within the plant.. The results showed that the 83 strains were divided into three groups at the similarity of 0.83, and a certain correlation was observed between the FAFLP gene types and the serotypes. When source tracing was conducted at the similarity of 0.86, FAFLP exhibited a higher sensitivity than traditional serotyping, and the result was consistent with the previous data on the prevalence rate obtained from a previous epidemiological investigation. The source tracing analysis showed a serious cross-contamination of Salmonella among animals, which occurred through the feces and fur. Furthermore, some Salmonella strains isolated from the feces and fur crossed the protective barrier of the processing plants and infected the downstream carcasses. Hence, processing plants should pay more attention to standardized pre-slaughter management strategies to avoid the cross-contamination problems during in-plant interventions. Additionally, in-plant interventions should include extra critical control points to reduce the risk of Salmonella infection.
