The optimized Liu kinetic equation was used to fit the adhesion process of Staphylococcus aureus biofilm. The results showed that culture temperature and pH as well as inoculum concentration influenced the adhesion process. The adhesion equilibrium of S. aureus biofilm was reached at approximately 60 min. Maximum microbial attachment was achieved at 35 ℃ and pH 7. Additionally, higher inoculum concentration led to increased microbial attachment. The Gompertz model was used to fit the growth and maturation process of the biofilm. The S. aureus biofilm growth curve showed a typical S type, with apparent lag, logarithmic, and stationary phases. This was different from the growth curve of suspended bacteria, which has a relatively short logarithmic phase. The adjusted coefficient of determination R2 (Adj) for the S. aureus biofilm models for adhesion, growth, and maturity were > 0.97, indicating that the Liu kinetic equation and the Gompertz model were suitable to describe the relationship between the external environment and S. aureus biofilm attachment as well as between growth and maturity, respectively.
