Effect of Various Extraction Methods on the Antioxidant Activity of Superoxide Dismutase from Rhodobacter sphaeroides
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Abstract:
Superoxide dismutase (SOD) activity levels were determined for the samples extracted from the biomass of Rhodobacter sphaeroides by grinding, ultrasonic extraction, enzyme assisted ultrasonicextraction, and nanomilling. The in vitro antioxidant activity of SODs extracted by the four methods from R. sphaeroides 3757 wet biomass were evaluated via evaluating their reducing power and scavenging capacity against ABTS+?, ?OH, and O2-?. The in vitro antioxidant activity of SODs extracted by ultrasonic approach and nanomilling from R. sphaeroides 3757 dry biomass were compared based on scavenging capacity against ?OH and O2-?. SODs extracted from wet and dry biomass of R. sphaeroides 3757 by nanomilling exhibited activities of 36.90 ± 1.25 U/g and 214.20 ± 7.72 U/g, respectively, which were higher than those of any other extraction method. The SOD antioxidant activity observed for nanomilling from wet biomass of R. sphaeroides 3757 was higher than that of grinding, ultrasonic extraction, and enzyme assisted ultrasonic extraction . For nanomilling, the reducing power was 11.3 ± 0.35 and the scavenging capacities against ABTS+?, ?OH, and O2-? were 92.1 ±3.32%, 76.90 ± 2.67%, and 36.90 ± 1.22%, respectively, when the solid/liquid ratio was 1:15. The antioxidant activity of SOD extracted by nanomilling from dry biomass of R. sphaeroides 3757 was higher than the counterpart extracted by the ultrasonic method. For nanomilling, when solid/liquid ratio was 1:20, the scavenging capacities against ?OH and O2-? were 57.10 ± 1.96% and 79.40 ± 2.71, respectively. The SODs extracted by different methods from both wet and dry biomass of R. sphaeroides 3757 exhibited significant antioxidant activity in vitro in a concentration-dependent manner. These results showed that SODs from R. sphaeroides 3757 exhibit potent in vitro antioxidant activity, and that nanomilling is the optimal extraction method.
