Molecular Identification of the Genotype of Staphylococcus aureus Biofilm
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Abstract:
Typical foodborne Staphylococcus aureus (SA) was selected to investigate microbiological safety. The biofilm-forming ability of 58 SA strains was quantitatively determined using crystal violet staining of biofilm. The biofilm-associated SA genes including those associated with regulation (icaA, icaD, and icaBC) and adhesion property (agr, atlE, and aap) were studied. The result demonstrated that all strains tested formed biofilms, which were strongly adhesive in three (5.2%), moderately adhesive in 23 (39.7%) and weakly adhesive in 32 (55.2%) strains. The icaA gene was amplified from 48 isolates and icaD and icaBC were amplified from 56 and 57 strains, respectively. The agr, aap, and atlE genes were amplified from the genomes of 56, 53, and 57 isolates, respectively. The results indicated that the ica operon is required for SA biofilm formation, the aap gene may act as an independent factor for promoting SA biofilm formation, while the atlE and agr genes are necessary regulatory factors for biofilm adhesion.
