High-throughput Sequencing Reveals Bacterial Structure in the Mud Pits of Heavy-fragrance Baijiu
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Abstract:
High-throughput sequencing was used to study the composition of the microbial community within the mud pits of differently-aged, heavy-fragrance Baijiu cellars in northern using the Roche GS Junior high-throughput sequencing platform. The results showed that the main bacterial taxa in the six mud pits tested included 79% Firmicutes, 4.9% Actinobacteria, 4.1% Bacteroidetes, 3.5% Lentisphaerae, 2.5% Synergistetes, and 6% others. With increasing age of the cellar, the Clostridiales Family XI. Incertae Sedis showed a decreasing trend, while the Actinobacteria and Synergistetes taxa showed an increasing trend. The Haloplasmataceae and Clostridiaceae taxa were detected only in the mud pits of a 30-year-old cellar, while the Syntrophomonadaceae taxon was detected in the mud pits of all 5-year-old cellars. The Lactobacillaceae and Porphyromonadaceae taxa were the dominant bacterial groups in only one 30-year-old cellar mud pit.
