The Effect of Phospholipase C on the Membrane Permeabilization of Escherichia coli
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Abstract:
The phospholipase C proteins from Clostridium perfringens（Cp-PLC）and Listeria monocytogenes (lm-PLC) were cloned and expressed in E. coli BL21 (DE3), and the effect of PLC on the permeability of E. coli BL21 membranes was investigated systematically. The recombinant PLCs expressed in C. perfringens and L. monocytogenes clearly affected the permeability of the inner membrane In contrast, the permeability of the outer bacterial membrane increased slightly. In addition, the effect of PLC enzymes incorporation on the permeabilization of E. coli BL21 membranes was tested, indicating that the addition of PLC enzymes markedly increased the permeabilization of the outer membrane. However, the permeabilization of bacterial inner membrane was not affected to the same extent. More importantly, coexpression of Cp-PLC and lm-PLC in E. coli BL21 to increase permeabilization of the host cell membrane opens a promising strategy for enhancing recombinant protein expression.
