Protective Effect of Cod Skin Collagen Peptides on Acute Liver Injury in Mice
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Abstract:
The protective effects of cod skin collagen peptides (CSCPs) on CCl4-induced acute liver injury in mice was examined. The acute toxicology dose and the maximal CSCP dose were determined. An acute liver injury model was established by intraperitoneal injection CCl4 in mice. The activity levels of serum ALT and AST were determined and the GSH-Px, SOD activities, and MDA content in liver homogenates were measured. Liver tissue samples of the mice were stained with hematoxylin and eosin (HE) to observe the effect of CSCP on mouse liver histopathology. Using TEM, the protective effects of CSCP on acute liver injury were examined. Expression of the Bax, Bcl-2, Cleavage Caspase-3, and TNF-α proteins were measured by western blot analysis. For the maximal CSCP dose of 8.0 g/kg, no mice died, indicating that CSCP was safe. ALT and AST activity levels in the serum samples were significantly lower in the CSCP group than the control group, and the maximal decrease was greater than 60%. Additionally, SOD and GSH-Px activity levels in liver homogenates increased significantly by 23% and 29%, respectively. The MDA content in liver homogenates significantly declined and the maximal decrease was 38%. Mouse liver tissue stained with HE revealed improvements in liver histology. The liver microstructure improved substantially based on transmission electron microscope (TEM) observations. Expression levels of Bcl-2 were higher, while the expression levels of Bax, Cleavage Caspase-3, and TNF-α were lower in the CSCP group than the control group. Taken together, CSCP effectively protects against acute liver injury by CCl4 in mice.
