Effects of Goose Bone Collagen and Peptides on Bone Marrow Mesenchymal Stem Cell Proliferation and Differentiation into Osteoblasts
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Abstract:
The effects of collagen, collagen containing calcium, collagen peptides, and collagen peptides containing calcium extracted from goose bones on the proliferation of rat bone marrow mesenchymal stem cells (BMSCs) and their differentiation into osteoblasts (OBs) were studied, and the mechanism by which collagen and peptides influence osteoporosis was described. The effects of goose bone collagen and peptides on BMSC proliferation were determined by an MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay. The osteogenic differentiation capacity of BMSCs was measured by alkaline phosphatase (ALP) staining, ALP activity, Alizarin Red staining, and osteocalcin secretion. The experimental groups exhibited greater proliferation of BMSCs than the control group. The positive ALP staining rate of the experimental group on day 14 was significantly higher that of the control group (p < 0.05); ALP activity levels of the experimental group on days 7 and 14 were significantly higher than that of the control group (p < 0.05). On day 21, the number of calcified nodules of the experimental groups, detected by Alizarin Red staining, was significantly high than that of the control group (p < 0.05). The osteocalcin secretion of experimental groups was significantly higher than that of the control group. These results indicated that macromolecular collagen, macromolecular collagen containing calcium, small collagen peptides, and small collagen peptides containing calcium promote BMSC proliferation and the differentiation of BMSCs into OBs, and the small collagen peptides containing calcium displayed the strongest effect.
