Determination of Trehalose in Saccharide Mixtures Based on Trehalase-catalyzed Hydrolysis
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
Based on the specificity of trehalase in hydrolyzing trehalose to glucose, a simple and rapid method for the determination of trehalose in saccharide mixtures was established. Excess trehalase ensured that trehalose would be hydrolyzed completely into glucose in a short time period, and then, the dinnitrosalicylic acid (DNS) method was used to determine the reducing sugar content in both samples before and after hydrolysis. The trehalose content could be calculated using the difference in reducing sugar content. The data of trehalose content in mixed solutions containing a trehalose standard, maltose, fructose, glucose, and sucrose were analyzed, and the results showed that the recovery rates of trehalose were in the range of 91.12%~105.92% and relative standard deviation was 2.22%~3.43%. Other saccharides in the mixture did not interfere with the content data with a detection limit of 70 μg/mL. The trehalose content in the intermediate product of fermentation was determined via the trehalase hydrolysis method and high performance liquid chromatography (HPLC). A t test showed that there was no difference between the two groups. The trehalase hydrolysis method was rapid, accurate, and sensitive. It can be used for samples with complex sugar compositions, and has a wide range of applications.
