Effect of Ultra-high Pressure on the Physicochemical Properties of Tilapia Actomyosin
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Abstract:
Changes in the physicochemical properties of tilapia actomyosin under ultra-high pressure (100, 300, and 500 MPa for 15, 30, and 45 min) were investigated. The results revealed that an increasing pressure and a longer holding time could reduce the solubility of tilapia actomyosin, and the lowest solubility (89.17%) was observed with 500 MPa treatment for 45 min, indicating that ultra-high pressure could promote protein accumulation and denaturation. The Ca2+-ATPase activity of tilapia actomyosin was lost under ultra-high pressure, suggesting the degeneration of myosin. As the pressure and holding time increased, the surface hydrophobicity of actomyosin also increased, and the highest hydrophobicity was achieved with 300 MPa treatment for 45 min, indicating that ultra-high pressure allowed more hydrophobic groups to be exposed. The total sulfhydryl content of actomyosin was reduced with increasing pressure, and the lowest value was reached with the 500 MPa treatment for 30 min; the disulfide bond content increased, and the highest level was achieved with the 300 MPa treatment for 45 min, indicating that the sulfhydryl group of actomyosin underwent oxidation to form disulfide bonds. These changes in the physicochemical properties demonstrate that the conformation of tilapia actomyosin changes after ultra-high pressure processing.
