Enhancement of β-1,3-glucanase Production in Salt-tolerant Zygosaccharomyces rouxii by Exponential Fed-batch
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Abstract:
High salt-tolerant Zygosaccharomyces rouxii A (tolerant of 24% NaCl) was used to produce ?-1,3-Glucanase in 10 L fermenter. Glucose (YEPD) is the optimum carbon source for yeast growth and enzyme production. Fermentation efficiency with YEPD batch culture is significantly higher than that with glycerol (YEPG) and ethanol (YEPE), respectively. Meanwhile, batch culture efficiency with acetate as carbon source was poor. The growth efficiency (biomass), the largest ?-1,3-Glucanase and enzyme productivity with YEPD batch cultivation was increased by 1.89% and 29.88%, 114% and 19.65%, 188% and 33% than that with YEPG and YEPE batch culture, respectively. Compared with the YEPD batch cultivation, exponential fed-batch with YEPF medium from 15 to 23 h, the time of maximum biomass was shorten by 12 h and the maximum biomass was increased by 19.29%. Moreover, ?-1,3-glucanase was nearly logarithmically synthesized to the largest enzyme activity (44.99 U/mL), the time of synthesis was shortened by 6 h and enzyme productivity was up to 2.14 U/(mL?h) that was increased by 76.86%. Exponential fed-batch model for effective enhancement of biomass and β-1,3-glucanase production by Z. Rouxii A was confirmed by the results above, which laid the foundations for the effective production of high salt-tolerant Z. Rouxii A, ?-1,3-glucanase products and the application of them in highly active fermentative function food industry.
