Identification of Prodigiosin-producing Serratia marcescens HFUT1301 Strain Isolated from Mandarin Fish Intestine
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
Prodigiosin, a pigment with anti-cancer effects, is produced by Serratia marcescens. A strain producing a red pigment was screened from the intestinal canal of mandarin fish and identified on the basis of colony morphology, physiological and biochemical characteristics, and 16S rDNA analysis. The structural characteristics of the red pigment was determined using ultraviolet-visible (UV-Vis) spectrophotometry, liquid chromatography-mass spectrometry (LC-MS), and Fourier transform infrared spectroscopy (FT-IR). The results showed that it appeared round colonies on basic medium, the diameter was within 1 to 3mm, the middle of the colony was red and opaque with a slight bulge in the center, and with smooth edges, while 16S rDNA fragment (1445 bp in size) showed 99% similarity with S. marcescens. This isolated strain was designated as S. marcescens HFUT1301. The purity of the red pigment exceeded 95% after ultrasound-assisted ethanol extraction and silica gel column chromatography. In pH 3- and pH 10-methanol solutions, there were significant absorption peaks at 535 and 470 nm, respectively. LC-MS showed that the main ion peaks were 323.5486 and 324.8468. The main absorption wavenumbers for FT-IR were 3396, 2923, 2851, 1710, 1465, and 1164 cm-1. Red pigment production reached 3.22 g/L in the fermentation medium. Based on previously reported structural characteristic of prodigiosin, the red pigment produced from S. marcescens HFUT1301 was speculated to be prodigiosin.
