Inhibitory Effects of Citral and Octanal Mixture on Penicillium digitatum
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
The effects of citral, octanal, and citral/octanal mixture (4:1, V/V) on the mycelial growth of Penicillium digitatum were investigated by using the agar dilution method. In addition, preliminary investigation was performed on the corresponding inhibitory mechanism of citral/octanal mixture against P. digitatum. Results showed that the citral/octanal mixture significantly inhibited mycelial growth of P. digitatum, with a minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) of 2.50 μL/mL for both. In contrast, the MIC and MFC of citral were 2.50 and 5.00 μL/mL, respectively, and those of octanal were 0.63 and 2.50 μL/mL, respectively. Scanning electron microscopy (SEM) showed that citral/octanal mixture treatment resulted in severe collapse and multiple folds on the hypha surface, causing irregular distortions in the structure and destroying cell integrity. Moreover, extracellular pH, extracellular conductivity, and absorbance at 260 nm significantly increased after treatment with citral/octanal (4:1, V/V). These results indicated that the citral/octanal mixture could destroy cellular integrity and increase the cell membrane permeability of P. digitatum, thereby causing leakage of cellular components, leading to the death of P. digitatum.
