Selenoarginine was added to the cultivation material to produce selenium-enriched Pleurotus ostreatus and the effects on the quality of its fruiting body were investigated, in terms of selenium-accumulation ability, glutathione peroxidase (GSH-PX) activity, volatile compound content, protein content, and protein distribution. The results showed that selenium concentration in the fruiting bodies had a significantly positive correlation (R=0.9967) with that in the cultivation material. Within the same cultivation material, selenium concentration in the cap was higher than that in the fruiting body. A larger amount of selenium was accumulated in proteins than in the polysaccharides. However, there were no significant changes in protein content and glutathione peroxidase activity. Gas chromatography-mass spectrometry (GC-MS) analysis indicated that selenium-enriched Pleurotus ostreatus had a simple composition of volatile compounds, with a relatively high alcohol content, than that of the non-enriched Pleurotus ostreatus. However, the relative content of 1-octen-3-ol in selenium-enriched Pleurotus ostreatus was higher than that of the non-enriched Pleurotus ostreatus. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis indicated that selenium did not change the distribution of proteins in the fruiting bodies of Pleurotus ostreatus.
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