The Effect of Mulberry Polyphenol Extract and Mulberry Wine Polyphenol Extract on Proliferation and Differentiation of BAT-cMyc Cells
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Abstract:
The effects of mulberry polyphenol extract (MPE) and mulberry wine polyphenol extract (MWPE) on the proliferation and differentiation of brown adipose tissue (BAT)-cMyc cells were investigated in this study. After treatment of BAT-cMyc cells with different concentrations of MPE and MWPE for 48 hours, the cells were collected, the growth inhibition effect of MPE and MWPE on BAT-cMyc cells was determined by MTT assay, and the IC50 values were calculated. The relative expressions of genes associated with cell proliferation, such as Ki67 and proliferating cell nuclear antigen (PCNA), were detected by real-time polymerase chain reaction (PCR). BAT-cMyc cells were then treated with MPE and MWPE until the sixth day of differentiation. Cells were collected and the efficiency of adipocyte differentiation was measured by Oil Red O staining and real-time PCR. The results showed that the IC50 value of BAT-cMyc cells was 186.37 μg/mL for MPE and 158.78 μg /mL for MWPE. Furthermore, growth of BAT-cMyc cells was inhibited in a dose-dependent manner. MPE and MWPE had no effect on BAT-cMyc cell proliferation, but could significantly increase the efficiency of the differentiation of BAT-cMyc cells into adipocytes and upregulate the expression levels of brown adipocyte differentiation-related genes, such as AP2 and C/EBPδ, during brown adipogenesis.
