Growth Kinetic Parameters of Multi-drug and Single-drug Resistant Vibrio parahaemolyticus Strains in Pure Culture and in Penaeus vannamei
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Abstract:
The variations in growth kinetic parameters of single- and multi-drug resistant Vibrio parahaemolyticus strains were investigated, and the relationship between bacterial resistance and risk was explored. A Bioscreen-C automated growth curve analysis system combined with the serial dilution method was employed to compare and analyze the maximum specific growth rates (μmax) of V. parahaemolyticus strains with varying degrees of drug resistance cultured in trypticase soy broth (TSB). Furthermore, Penaeus vannamei was inoculated with the VPD18 strain, which is resistant to six antibiotics, and the VPD43 strain, which is only resistant to ampicillin. Growth curves at different temperatures were established and then the growth dynamics parameters were calculated using the Baranyi model. There was no significant difference in μmax values between the single- and multi-drug resistant strains grown in TSB (P > 0.05). However, in P. vannamei, the μmax of the single-drug resistant strain was higher than that of the strain resistant to six antibiotics, but the latter strain had a shorter lag time (λ). These results suggest that the increase of antibiotic resistance may lead to a shortened λ, but has no significant effect on the μmax. The results of this study can provide basic data for the development of a quantitative risk assessment of V. parahaemolyticus in China.
