Mutation Screening to Detect Strains that Produce Bacterial Cellulose at High Yield by N+ Ion Beam Irradiation
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Abstract:
In order to obtain strains that produce a high yield of bacterial cellulose (BC) and improve the properties of BC, N+ ion beam irradiation was used to conduct mutation screening in Acetobacter xylinum. A plate-96-well plate-cuvette screening system was established, in which parameters such as the size, smoothness, density, wettability and rate of the colony formation, pliability, and yield of the BC film were chosen as the screening criteria. Two target strains, 5D and 2D, were obtained that could produce BC with improved product properties at an enhanced yield. The BC yields of 5D (4.84 g/L) and 2D (4.53 g/L) were 89.06% and 76.95% higher than that of the original strain, respectively. Fourier transform-infrared spectroscopy, X-ray diffraction, scanning electron microscopy, and a tensile test were used to study the properties of BC. The results showed that the BC produced by the target strains had higher crystallinity and tensile strength, a smaller elongation at break, a denser layered structure, and a clearer three-dimensional network structure compared with the product from the original strain, indicating that they had superior mechanical properties. The success in obtaining the target strains lays a foundation for improving the BC yield and properties.
