In Vitro Antioxidant Activity of Abalone Viscera Hydrolysates Isolated by Ultrafiltration
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Abstract:
Abalone viscera hydrolysates were separated by ultrafiltration, and the chemical composition and antioxidant activity of the hydrolysates in different ranges of molecular-weight were investigated. Four fractions (FA, FB, FC, and FD) were obtained by membrane ultrafiltration with molecular weight cut-off (MWCO) of 10000, 3000, and 500 u. In fraction FA, total sugar and protein contents were 34.06% and 17.20%, respectively. However, fractions FB, FC, and FD were mainly composed of proteins, and the free amino acid content was 23.20, 25.47, and 28.68 g/10 g, respectively. Based on the results from tricine-sodium dodecyl sulfate polyacrylamide gel electrophoresis and β-elimination, the high molecular-weight fraction (HMWF) of FA in the spacer gel contained glycoproteins with N-glycosidic linkage, and components with molecular weight of 25 ku were proteins. Gel permeation chromatography data indicated that molecular weight of the fractions FB, FC, and FD were each approximately 300 u. Additionally, the result of in vitro antioxidant assay indicated that fraction FA had a relatively strong 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging ability and reducing power at low concentration. When the concentration was increased above 5 mg/mL, there were no significant differences in the DPPH radical-scavenging abilities among these fractions, but the reducing power of fraction FA was lower than that of other fractions. In both, low and high concentrations, the hydroxyl radical-scavenging abilities of fractions FB, FC, and FD were stronger than that of FA.
