Microbial Transformation of Polydatin by Endophytic Fungi Isolated from Polygonum cuspidatum and Antioxidant Activity of the Products
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
The microbial transformation of polydatin by endophytic fungi isolated from Polygonum cuspidatum and the antioxidant activity of the transformed products were investigated. Screening of endophytic fungi for microbial transformation of polydatin was carried out using thin-layer chromatography (TLC). Fungi were identified by morphological characteristics and analysis of the ITS-5.8S rDNA region. Polydatin transformation products were isolated and purified by macroporous adsorption resin, preparative TLC, and high-performance liquid chromatography. Using vitamin C (Vc), BHT, polydatin, and five types of polydatin derivatives as standard substances, the DPPH? and OH? radical-scavenging capacities of transformation products were investigated. An endophytic strain J3, capable of transforming polydatin was identified preliminarily as Aspergillus fumigatus (Genbank accession number: KM434883) by morphological characteristics and analysis of the ITS-5.8S rDNA region (573 bp). The transformed product, A-2, showed DPPH? and OH? radical-scavenging capabilities in vitro, while the OH? radical-scavenging ability was higher than that of substrate polydatin. However, transformed product, A-1, did not show DPPH? and OH? radical-scavenging capabilities in vitro. Endophytic fungus Aspergillus fumigatus isolated from Polygonum cuspidatum was thus shown to transform polydatin into products with antioxidant property.
