Isolation and Identification of the Antiviral Active Ingredients of Inonotus obliquus
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Abstract:
The chemical composition, structure, and antiviral activity of ethyl acetate extracts of Inonotus obliquus was identified and analyzed. Inonotus obliquus is an edible and medical fungus with diverse and complex structures and a wide range of biological activities; in addition, this fungus has a low toxicity to humans and is rich in secondary metabolites. Inonotus obliquus fruit bodies were dried, ground, and sequentially extracted at room temperature using ethyl acetate, methanol, and water. Ten compounds were isolated from the ethyl acetate extract of Inonotus obliquus using various chromatographic techniques. Various modern spectroscopic methods of analysis and the data available in literature were used to identify the following compounds: betulin (1), inotodiol (2), 3-hydroxy-lanosta-8,24-dien-21-al (3), 3β-hydroxytirucallic acid (4), ergosterol-5,8-peroxide (5), 3β-hydroxy-5α,8α-epidioxyergosta-6,9,22-triene (6), di(n-butyl) phthalate (7), 5-dehydroergosterol (8), pentacosanoic acid (9), and lignoceric acid (10). Among these, compounds 6 and 7 were isolated from Inonotus obliquus for the first time. Furthermore, the cytotoxicity and anti-HSV-1 activity of the isolated compounds 1–8 were also investigated. Compound 3 showed the strongest anti-HSV-1 activity, with a half maximal inhibitory concentration (IC50) of 98 μM; on the other hand, compound 7 exhibited the strongest cytotoxicity, with a 50% cytotoxic concentration (CC50) of 24 μM. This study provides a scientific basis for the future development of anti-HSV drugs from Inonotus obliquus.
