Development and Properties of a Polypyrrole-based Electrochemical Biosensor for Cholesterol Detection
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
A novel, current-based cholesterol biosensor was produced by electrochemical polymerization of pyrrole monomers, using cholesterol oxidase (ChOx) and horseradish peroxidase (HRP) as catalysts. Polypyrrole (PPY) was prepared by electrochemical polymerization and the microstructure of the PPY film was characterized by scanning electron microscopy (SEM). The electrochemical characteristics and anti-interference capacity of the biosensor were evaluated by cyclic voltammetry (CV). The results of this analysis revealed that the PPY film obtained after 300 polymerization circles had optimum sensor performance. The biosensor displayed optimal operation at a pH of 8.0 and showed good detection performance at cholesterol oxidase and horseradish peroxidase concentrations of 30 and 20 mg/mL, respectively. The biosensor displayed a linear range of 2.0 × 10-6 to 1.6 × 10-4 mol/L and an apparent Michaelis-Menten constant ( ) of 5.6 × 10-6mol/L. The biosensor also exhibited excellent anti-interference activity after the addition of glucose, ascorbic acid (AA), and uric acid (UA), which affected the current by 8.1%, 8.5%, and 10.8%, respectively. The cholesterol biosensor exhibited good selective response to cholesterol, and can, therefore, be used in the determination of cholesterol content.
