Impact Mechanism of Staphylococcus aureus Genomic Islands on Multi-drug Resistant Phenotype and Biofilm-forming Capacity
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Abstract:
Staphylococcus aureus (S.aureus) has been long recognized as a common food-borne pathogen in food safety. To study their drug resistence, genomic island typing and biofilm-foming capacity, a toal of 127 clinical isolates of staphylococci strains from Guangzhou were subject to the investigation. A detection of Staphylococcus aureus was conducted with a Micro-broth dilution method to determine the strain susceptibility results from 26 kinds of antibiotics. S.aureus was further confirmed with the detection of specific 16S rRNA, femA, mecA genes by PCR. Ccr and mec complexes of S.aureus SCCmec genomic island elements were typed by multi-PCR. In this experiment, 107 drug-resistent staphylococci are all multi-drug resistant strains, and 76.1% (86/113) show resistance to nine kinds of antibiotics or more. 113 Staphylococcus SCCmec typing results: typeI (0 strains), typeII (12 strains), typeIII (73 strains), typeIV (10 strains), typeV (11 strains),5strains is not typing. In this paper, the correlation of genomic islands with Staphylococcus aureus drug-resistance phenotype and biofilm-forming capacity is analyzed and discussed. It provides a research base for further security control on a variety of food contamination caused by food-borne microorganisms.
