Evaluation of the Anti-proliferative Activities of Major Fruits in China on Human Hepatic Carcinoma (HepG2) and Colon Adenocarcinoma (Caco-2) Cells
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
The total phenolic content and the anti-proliferative activities of the phenolic extracts against HepG2 and Caco-2 cells were determined by the Folin-Ciocalteu method and the methylene blue assay, respectively; the correlation between the total phenolic content and the anti-proliferative activity of the extracts was analyzed. The results of these analyses revealed that plum (Prunus salicina Lindl.) had the highest total phenolic content (1686.08 ± 96.94 ?mol gallic acid equivalent (GAE)/100 g of fresh fruit), whereas watermelon (Citrullus lanatus) (83.54 ± 2.10 ?mol GAE/100 g of fresh fruit) and hami melon (Cucumis melo var. saccharinus) (79.35 ± 0.76 mol GAE/100g of fresh fruit) had the lowest phenolic content among the 25 tested species. Among the fruit species with quantifiable anti-proliferative activities (half maximal effective concentration (EC50)), plum and guava (Psidium guajava Linn.) showed the strongest anti-proliferative activities against HepG2 cells (EC50 values: 18.99 ± 0.06 and 20.94 ± 0.09 mg/mL, respectively), whereas pears (Pyrus spp.) showed the weakest anti-proliferative activity against these cells (EC50 value: 389.63 ± 10.82 mg/mL). Plum also demonstrated the strongest anti-proliferative activity against Caco-2 cells (EC50 value: 8.73 ± 0.11 mg/mL), while the activity of pitava (Hylocereus undulatus Britt.) was observed to be the weakest (EC50 value: 388.07 ± 7.04 mg/mL). The anti-proliferative activities of fruits on HepG2 and Caco-2 cells were significantly correlated to their total phenolic content (R2 = 0.4147, p < 0.01; R2 = 0.4071, p < 0.05, respectively). This correlation indicates the high anti-proliferative effect of fruit polyphenols on cancer cells.
