The Apoptosis-inducing Effects of Kuding Tea Flavonoids on Human Oral Cancer HSC-3 Cells Through Caspase Activation
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Abstract:
The apoptosis-inducing effects of Kuding tea flavonoids on human oral cancer HSC-3 cells through caspase activation (cysteinyl aspartate specific proteinase) were observed in this study. The MTT assay showed that Kuding tea flavonoids inhibited the growth of HSC-3 cancer cells at a concentration of 0~150 μg/mL; however, the flavonoids did not influence the proliferation of human oral keratinocyte cells (normal cells). Kuding tea flavonoids (concentration, 150 μg/mL) showed a better growth inhibitory effect (78.9%) on HSC-3 cancer cells compared to the soybean isoflavones capsule substance (42.8%) available in the market. Analysis of the mRNA expression in cancer cells showed that Kuding tea flavonoids upregulated caspase-3, caspase-8, caspase-9, Bcl-2 associated X protein (Bax), factor associated suicide (Fas), and Fas ligand (FasL) mRNA expressions, and downregulated the expressions of B-cell lymphoma-2 (Bcl-2), Bcl-xL, cellular inhibitor of apoptosis protein-1 (cIAP-1), and cIAP-2 mRNA, compared to the control. This increase and decrease in mRNA expression was observed to be correlated to the increase in concentration of Kuding tea flavonoids. These results demonstrated that Kuding tea flavonoids induced in vitro apoptosis in HSC-3 cancer cells via caspase activation.
