Extraction of Phenolic Acids and Flavonoids from Nidus Vespae Using Silica-supported Ionic Liquid-based Matrix Solid-phase Dispersion
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Abstract:
In this study, a new kind of silica-supported ionic liquid (S-SIL) was prepared by impregnation and employed as the dispersion adsorbent for matrix solid-phase dispersion (MSPD). This system was used for the simultaneous extraction of caffeic acid, ferulic acid, morin, chrysin, and kaempferide from Nidus Vespae (honeycomb). High-performance liquid chromatography (HPLC) was used to separate and identify the analytes. The experimental conditions for S-SIL-based MSPD were optimized. S-SIL containing 10% [C6MIM]Cl was used as dispersant, 20 mL n-hexane was used as washing solvent, and 15 mL methanol was used as the eluent. The mass ratio of sample to S-SIL was set as 1:4. The calibration curve of each target compound showed a good linear relationship within the linear range (r > 0.9995). The limits of detection and quantification were in the range of 0.05 to 0.18 μg/mL and 0.17 to 0.59 μg/mL, respectively. The relative standard deviations (RSDs) of intra-day and inter-day precision were lower than 5.48% and 6.60%, respectively. The recoveries were between 83.73% and 95.32%. Thus, a novel method of S-SIL-based MSPD combined with HPLC was developed in this study, to identify five phenolic acids and flavonoids in Nidus Vespae. Compared with other extraction methods, this method required less extraction time, sample, and organic solvent, because it combined the advantages of ionic liquid and MSPD. This method could be widely applied for the extraction of phenolic acids and flavonoids from medicinal plants and animals.
