Effect of Fish Skin Collagen Peptides on Cell Viability of Human Skin Keratinocytes
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Abstract:
This study investigated the effects of shark skin collagen hydrolysates (SH), tilapia skin collagen hydrolysates (TH), and their isolated fractions on the viability of human skin keratinocyte (HaCat). The HaCat cell viability, determined with the MTT method, was increased notably with the addition of 0.05 mg/mL or 0.20 mg/mL SH during the exponential growth phase. However, when the concentration of the added SH was increased to 1.00 mg/mL, no significant proliferation of HaCat was observed. The HaCat cell viability with the addition of TH was higher than that of the control (without the addition of TH), but TH concentrations did not significantly influence the viability. On the other hand, the HaCat cell viability with addition of the fractions, which were isolated from SH or TH by Sephadex G-15, was much higher than that with the addition of dipeptide (Gly-Tyr) or Tyr. Compared to the sample with added GSH, the MDA content was lower and the SOD activity was higher with the added isolated fractions irrespective of fish skin collagen hydrolysates. Based on these results, it was concluded that the fractions isolated from fish skin collagen hydrolysates by Sephadex G-15, can effectively promote the proliferation of HaCat and have great potential to replace GSH as antioxidative peptides in the cosmetics industry.
