Cloning and Bioinformatics Analysis of Fe-SOD Gene in Alicyclobacillus acidoterrestris
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Abstract:
Alicyclobacillus acidoterrestris, a genus of acidophilic and thermophilic resistant bacteria, is an important microbial resource of heat and acid resistant enzymes, a variety of enzymes isolated from which have acid and thermal stability. Superoxide dismutase (SOD) is widely used in food, clinical treatment, and cosmetic industry for its important physiological functions. In order to deeply explore the molecular properties of SOD gene in Alicyclobacillus acidoterrestris, the Fe-SOD gene was cloned, sequenced and analysed using homologous cloning, thermal asymmetric interlaced PCR and bioinformatics. The results showed that Fe-SOD gene(GenBank accession number: JN614998) contains a complete ORF of 606 bp, encoding 202 amino acid, the deduced amino acid sequence shared the highest homology with Fe-SOD from A. acidocaldarius DSM 446 (78%). A. acidoterrestris Fe-SOD is composed of five motifs and the active site of SOD contains the residues of His27, His82, Asp164, and His168, which has characteristics of protein structures of Fe/Mn SODs family. Cloning and bioinformatics analysis of A. acidoterrestris Fe-SOD will provide information for constructing prokaryotic expression vector and obtaining the gene engineering bacteria strains of producing heat and acid resistant SOD.
