Structural Analysis of Polysaccharides from Pleurotus ferulae Lenzi
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Abstract:
The Pleurotus ferulae Lenzi polysaccharide (PFLP) was isolated and purified to study its components and structure. The relative molecular mass and distribution of PFLP were analyzed by high-performance gel filtration chromatography. The structure was analyzed by gas chromatography (GC), partial acid hydrolysis, periodate oxidation, Smith degradation, methylation, infrared spectroscopy (IR), 1HNMR, Congo red experiment, and iodine-potassium experiment. Two components, PFLP1 and PFLP2, were obtained through extraction and purification. The result indicated that the relative molecular mass and distribution of PFLP1 was 9400~9900 u. PFLP1 was composed of rhamnose, mannose, glucose, and galactose with a molar ration of 1:1.54:18.6:3.64. The relative molecular mass and distribution of PFLP2 was 9700–10400 u. PFLP2 was composed of rhamnose, xylose, mannose, glucose, and galactose with a molar ration of 1:1.08:0.65:6.76:4.28. Both PFLP1 and PFLP2 contained glycosidic bond of (1→2), (1→3), (1→6), and (1→4). PFLP1 and PFLP2 possessed typical infrared absorptions of polysaccharides, and the sugar chains were mainly pyranoside with β-configuration and contained a type of protein glycoconjugate. PFLP1 and PFLP2 possessed triple helical structures containing long side chains and branches.
