Stability of ERIC-PCR and Sau-PCR Techniques on Listeria monocytogenes Typing
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Abstract:
To evaluate the stability of enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) and Sau-PCR for typing of Listeria monocytogenes (LM), five isolates of LM from a local vegetable market in Guangzhou and a food processing plant in Xiamen were cultured. DNA was extracted from the cultures incubated at room temperature after 24, 48, and 72 h. In addition, DNA was extracted from the 5th, 10th, 15th, and 20th subcultures. ERIC-PCR and Sau-PCR were then used for typing and changes in fingerprint diagrams with time and increase in the number of generations in subculture were determined. The results indicated that apart from some missing bands, there were no additional bands after room-temperature culture and subculture. However, there were minor changes in the overall band appearance. The homology of ERIC-PCR and Sau-PCR results was higher than 92% and 94%, respectively, indicating that the two typing methods were stable and had epidemiological significance for typing of LM cultures incubated for less than 72 h and 20 generations.
