Patterns of T-2 Toxin Residue in Prawns and Its Subsequent Effect on Genes in Male Mice
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Abstract:
This study examined the pattern of T-2 toxin residue in prawns following acute exposure, and the genetic alterations in mice following administration of the poisoned prawns. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to detect the content of residual T-2 toxin in prawns after intramuscular injection. The mice were administered the poisoned prawns by gavage. The results showed that the content of free T-2 toxin in prawns positively correlated with its increasing dose. T-2 toxin appeared to have accumulated in the prawns, and the amounts were significantly different among the various organs. The lowest content of free T-2 toxin was found in muscle; T-2 toxin content was close to zero. The sperm deformity and micronucleus rate of mice gradually increased with an increase in the exposure dose, compared to the control group (P < 0.05). The four organs of the prawns in the high dose group were toxic to the mice, and the toxicity levels were the highest in the muscles and hepatopancreas, followed by the intestinal tract. The results indicated that T-2 toxin in prawns could be easily concealed, especially in the muscle, where free T-2 toxin is converted to masked T-2 toxin. As a result, T-2 toxin could remain undetected, resulting in secondary harm to other organisms.
