UV-Induced Gene Expressions in Sea Cucumber (Stichopus japonicus) Body Wall
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Abstract:
In this study, gene expressions for UV-induced (58 μW/cm2, 30 min) body wall autolysis of sea cucumber (Stichopus japonicus) were investigated. Total RNA was extracted using the TRIzol method from sea cucumber body wall. Twenty-nine genes, including cathepsin C (CC), fibrinogen-like protein A (FGL), senescence-associated protein (SAP), major yolk protein 2 (MYP2), cathepsin L (CL), calreticulin, matrix metalloproteinases14 (MMP14), and acetylcholinesterase (AChE), were amplified using reverse transcription-polymerase chain reaction with cytochrome B (CytB) as an internal control. The results indicated that the gene expressions of CC, FGL, SAP, and MYP2 were significantly enhanced by 71.4 ± 44.8%, 27.1 ± 18.4%, 43.7 ± 21.6%, and 165.5 ± 122.7%, respectively, after UV irradiation for 30 min (p < 0.05). However, the gene expressions of CL, calreticulin, MMP14, and AChE did not change significantly. These results suggest that CC, FGL, SAP, and MYP 2 may be involved in the autolysis of sea cucumber body wall.
