The Effects of Three Ionic Species on the BSA Protein Release Properties of Chitosan/Pectin Polyelectrolyte Complexes
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Abstract:
Chitosan/pectin polyelectrolyte complexes (PECs) were prepared by adding NaCl, Na2SO4, and FeCl3, respectively. The bovine serum albumin (BSA) loading efficiency and simulated releasing behavior of PECs were studied and the effects of the three ionic species on the protein release properties of chitosan/pectin PECs were investigated. Results showed that the encapsulation efficiency of FeCl3 PEC was the highest, followed by Na2SO4 and NaCl; the loading efficiency of NaCl PEC was the highest, followed by Na2SO4 and FeCl3. In single simulation, the BSA accumulative release ratio in the GI tract juices of PECs was the lowest; however, it was the highest in simulated colon juice, The BSA release ratio of PEC was reduced by the addition of NaCl, while the addition of Na2SO4 and FeCl3 increased the BSA release ratios. The Fick diffusion theory can be used to describe the kinetics of the BSA release behavior of PECs with the three ions. In successive simulations, the BSA accumulative release ratio of PECs in simulated GI tract juices was the lowest, while BSA was released in large quantities in the simulated colon, with NaCl PEC having the lowest accumulative release ratio. The BSA accumulative release ratio of PECs was more than 98% when pectinase was present in simulated colon juice. The release behavior of PECs s was the Non-Fick diffusion type. All the chitosan/pectin PECs formed with the three ionic species showed favorable pH sensitivity and site-specific delivery properties.
