Protective Effects of Grape Seed Proanthocyanidin Extract (GSPE) on cis-Diamminedichloroplatinum (CDDP)-Induced Renal Oxidative Damage and Mitochondrial Injury in Rats
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Abstract:
In this paper, the protective effects of grape seed proanthocyanidin extract (GSPE) on cis-diamminedichloroplatinum (CDDP)-induced renal oxidative stress and mitochondrial injury in rats were investigated. Rats were randomly divided into five groups: normal control group, CDDP model group, GSPE (400 mg/kg) group, CDDP + GSPE (200 mg/kg) group, and CDDP + GSPE (400 mg/kg) group. Each group was administrated with distilled water or the corresponding doses of GSPE by gavage for 15 consecutive days. After 10 d of administration, the CDDP and CDDP + GSPE groups were administered a single intraperitoneal injection of CDDP (8 mg/kg), and the remaining groups were administered with normal saline by intraperitoneal injection. The blood urea nitrogen (BUN), creatinine (CrE), renal index, antioxidant index for renal cortex, and index for the carbohydrate metabolic enzyme were measured, and the renal structures were observed using a light microscope. Compared to the CDDP model group, the GSPE pretreated rats showed a significant decrease in renal index as well as BUN and CrE contents. The CDDP treatment improved the contents of renal cortical reduced glutathione (GSH), and malondialdehyde (MDA), meanwhile, the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-px), malate dehydrogenase (MDH), and hexokinase (HK) were enhanced. The pathologic slice revealed that GSPE pretreatment could reduce CDDP-induced pathological damage such as glomerular atrophy. This study suggested that GSPE had a protective effect on the CDDP-induced renal oxidative stress and mitochondrial injury in rats.
