Inhibitory Effect of Eucheuma gelatinae Polysaccharides on H22 Hepatoma Xenograft Cells
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Abstract:
In this study, the antitumor effect of Eucheuma gelatinae polysaccharides (EGP) on H22 transplanted solid tumor cells was investigated. Mice were orally administered EGP extracted by alkali treatment at room temperature. The tumor inhibition rate, immune organ index, proliferation ability of splenocytes (T and B lymphocytes), splenic NK cell killing activity, and phagocytosis by peritoneal macrophages were measured. Moreover, peripheral blood lymphocyte subsets were investigated by flow cytometry. The results showed that EGP significantly inhibited H22 hepatoma cell growth in mice, which were transplanted with tumor and gavaged with EGP. The tumor inhibition rate by EGP was 31.17% in the low-dose group [300 mg/(kg?d)] and 59.67% in the high-dose group [600 mg/(kg?d)] at the end of the experimental period. Compared with the model group, EGP significantly promoted splenocyte proliferation, killing activity of NK cells, and macrophage phagocytosis. In addition, the ratio of CD3+ to CD8+ T cells in the tumor-bearing mice was significantly increased (p < 0.05) in the high-dose EGP group. The results suggested that EGP improved immunity of tumor-bearing mice and exerted a remarkable anti-tumor activity.
