A Study of Flavonoids from the Leaves of Corylus heterophylla Fisch.
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Abstract:
Qualitative analysis of flavonoids in the leaves of Corylus heterophylla Fisch. was performed using high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS). Compounds were identified by comparison of retention times, parent ions, and secondary ion fragments. Quantitative analysis of the flavonoids was performed using HPLC-ESI-MS/MS in the multiple reactions monitoring (MRM) mode. The results showed that 12 compounds were found in the leaves of C. heterophylla, and 11 compounds were reported for the first time, namely epicatechin (41.45 ± 1.87 μg/g, dry weight), chlorogenic acid (3-caffeoylquinic acid, 389.00 ± 17.15 μg/g), syringetin-3-O-glucoside (6169.78 ± 209.73 μg/g), salvianolic acid C (443.37 ± 21.07 μg/g), chrysoeriol-6-C-glucoside (206.81 ± 10.07 μg/g), hyperoside (quercetin-3-O-galactoside, 154.44 ± 7.63 μg/g), quercitrin (quercetin-3- O-rhamnoside, 937.15 ± 40.86 μg/g), laricitrin-3-O-galactoside (17.27 ± 0.82 μg/g), malvidin-3-O-glucoside (202.74 ± 10.04 μg/g), laricitrin-3- O-glucoside (101.27 ± 4.99 μg/g), and kaempferol-3-O-rhamnoside (112.37 ± 4.77 μg/g).
