Molecularly Imprinted Solid-phase Extraction Membrane-high Performance Liquid Chromatography for the Determination of Triazole Fungicide Content in Grains
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Abstract:
A molecularly imprinted solid-phase extraction membrane (MISPEM) coupled with high-performance liquid chromatography (HPLC) method for the simultaneous determination of four triazole fungicides in grain samples was developed. Residues of bitertanol, triadimefon, diniconazole, and tebuconazole in grains were separated and enriched using the self-prepared MISPEM. These enriched residues were subsequently analyzed by HPLC. Process characteristics such as washing agents, the type and amount of eluents, and the detecting conditions were optimized. For this process, using 5 mL water and 3 mL methanol as the washing agent and eluent, respectively; the samples were separated with a C18 column, using a mobile phase of methanol and water (82:18, V/V). The separation was quantified using an external standard method, by detection at a wavelength of 210 nm. The average recovery rates of the four fungicides were discovered to be between 84.2% and 98.1%; the relative standard deviations (RSDs) ranged from 1.2% to 2.7% (n = 5). The MISPEM showed a good cleansing effect on the samples, as well as strong adsorption capacities for the four fungicides. These results suggest that this method can be used in the efficient, sensitive, and rapid determination of triazole fungicides in grain samples.
