Simultaneous Determination of the Contents of 16 Types of Monophenols in Wine, Fruit Wine, and Fruit Vinegar by RP–HPLC
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Abstract:
A new method based on HPLC was established for the simultaneous determination of the contents of eight types of phenolic acids, four types of flavan-3-alcohols, three types of flavonols, and one type of stilbene in natural fruit and vegetable products, including wine, fruit wine, and fruit vinegar. Chromatographic separation was performed using a Waters XBridgeTM Shield RP18 (4.6?250 mm, 3.5 ?m) column at 30 ℃ with an acetonitrile/acetic acid/water mixture as the mobile phase at a flow rate of 0.8 mL/min. The injection volume was 20 ?L; gradient elution was applied and a diode array detector (DAD) was used to monitor signals at 280 nm and 320 nm. All 16 monophenols were successfully separated and their contents showed good linear relationships with the peak areas. The method had a high recovery rate, good repeatability, good accuracy, and high speed. The contents of 16 types of monophenols in three wines, three fruit wines, and three fruit vinegars were determined using this method. The results suggested that fourteen, fourteen, and nine types of monophenols could be quantified in dry red wine, dry white wine, and rosé wine, respectively; seven, seven, and eight types could be quantified in apple wine, cherry wine, and pomegranate wine, respectively; eight, nine, and eight types could be quantified in apple vinegar, persimmon vinegar, and kiwifruit vinegar, respectively. The results indicated that the method was accurate and reliable, and was suitable for the analysis of different monophenols in various fruit and vegetable drinks including wine, fruit wine, and fruit vinegar.
