Influence of Nisin Compound Antimicrobial Agent on Microbial Diversity and Quality of Stored Wheat
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Abstract:
Wheat grain was treated with an antimicrobial agent prepared from various combinations of potassium sorbate, 2-aminobutane, and nisin, and then stored at 30 ℃ and 75% relative humidity for 35 days. The effect of the antimicrobial agent on the microbial diversity in the stored wheat and its correlation with the subsequent quality of wheat was studied. The result showed that fungistatic activity of nisin, nisin in combination with potassium sorbate, nisin in combination with 2-aminobutane, potassium sorbate in combination with 2-aminobutane, and nisin in combination with potassium sorbate and 2-aminobutane was 3.62, 64.34, 8.61, 37.03, and 50.02 times higher than that of the control, respectively. Moreover, the values for inhibitory activity of nisin in combination with potassium sorbate and 2-aminobutane on the microbial count of fungi, Aspergillus, Penicillium, Bacillus sp., and other bacteria were 50.02, 55.44, 2.89, 75.14, and 86.66 times higher than that of the control. The optimal ratio of the combination was 0.20 g Nisin, 1.00 g potassium sorbate, and 2.00 g 2-aminobutane per kilogram of wheat. The correlation analysis showed that the microbial count of fungi, Aspergillus, Penicillium, Bacillus sp., and other bacteria were positively correlated with the amount of fatty acids and the total acids, and negatively correlated with the precipitation value.
