Physicochemical Characteristics and in vitro Antioxidant Activity of Polysaccharide PMP-2a from Pleurotus geesteranus
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Abstract:
In this study, a water-soluble, crude polysaccharide was extracted from Pleurotus geesteranus and the mycelium polysaccharide was obtained by deproteinization, discoloration, and purification using diethylaminoethanol (DEAE)-Sepharose Fast-Flow ion-exchange chromatography and Superdex-200 column chromatography. The purity of the polysaccharide was determined using high-performance liquid chromatography (HPLC); Colorimetric measurement of the total sugar, uronic acid and protein content; and physicochemical properties and partial structural characteristics, studied by ultraviolet (UV), infrared (IR) and β-elimination reaction methods. The in vitro antioxidant activity of PMP-2a was determined by superoxide radical and hydroxyl radical scavenging assays. The results showed that PMP-2a was a homogeneous polysaccharide, consisting of Rha, Glc, Gal, Man, and Ara in a molar ratio of 1:13.18:2.19:7.23:3.15. The total sugar, uronic acid, and protein content of PMP-2a were 82.6%, 19.8%, and 10.6%, respectively; no nucleic acid was found. Typical absorption bands of polysaccharides and glycosidic bonds in α-D-pyranoside were found in the IR spectrum. The molecular weight was 9.28 × 104 Da, and the β-elimination reaction indicated that PMP-2a was linked to peptide chains through an -O- bond. PMP-2a showed significant superoxide radical- and hydroxyl radical-scavenging activities in a dose-dependent manner.
