Method to Determine the Triple-Helical β-glucan Content in Yeast
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Abstract:
A quick method to determine the triple-helical β-glucan content in yeast using Congo red was established. The final concentration of dimethyl sulfoxide, which was the cosolvent for the yeast triple-helical β-glucan in the measuring system, was 2%, and the maximum absorption wavelength of the Cogon red system was 523 nm. The regression equation for the β-glucan mass (Y) and ΔOD523 value (x) was Y = 923.0x - 5.641, with a coefficient of determination (R2) of 0.996. The β-glucan mass and ΔOD demonstrated a strong linear relationship in the mass range of 35~199 μg of β-glucan. High accuracy was achieved using the Congo red method to measure yeast triple-helical β-glucan, with a relative standard deviation (RSD) of 2.21%. Using this method, the content of triple-helical β-glucan in yeast cell wall samples was 18.33%. The recovery rate varied from 97.68% to 103.90% (average, 99.60%), and the RSD of the recovery rate was 2.32%. The results indicated that the Congo red method used to determine the triple-helical β-glucan content had a high recovery rate and was suitable to measure yeast triple-helical β-glucan. It was a rapid, simple, and efficient determination method that could meet the demands of the industrial production process.
