Inhibitory Effect of Xanthoceras sorbifolia Nutshell Saponin on Pancreatic Lipase
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Abstract:
In this study, the influence of saponin from Xanthoceras sorbifolia nutshell (SXS) which was considered as a weight-reducing agent on pancreatic lipase activity was studied. The effects of SXS purified by AB-8 macroporous resin on pancreatic lipase were investigated by evaluating various experimental conditions (pH, reaction time, and order of adding reagents). The mechanism of SXS-mediated inhibition of pancreatic lipase was studied using ultraviolet absorption spectrometry (200~340 nm) and fluorescence spectrometry (excitation wavelength 290 nm, scanning wavelength 300~450 nm). The results indicated that the inhibition rate of SXS to pancreatic lipase activity could reach 87.5% using the optimal reaction system of pH 7.4, reaction time 15 min, and preheating the substrate and SXS together prior to adding the pancreatic lipase solution. Following the reaction of SXS and pancreatic lipase, the microenvironment of luminous amino acids (Trp, Tyr), the molecular structure, and the conformation of pancreatic lipase were changed. This resulted in a red-shift in the maximum absorption wavelength in the ultraviolet absorption spectrum of pancreatic lipase, and a reduction in fluorescence intensity. Further, pancreatic lipase activity was inhibited. Thus, SXS can act as a weight-reducing agent through the inhibition of pancreatic lipase.
