Distribution and Antioxidant Activity of Free and Bound Phenolic Acids in Sugarcane Tissues
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Abstract:
In order to explore the different forms of phenolic acids in the tissues of sugarcane, qualitative and quantitative analyses of free and bound phenolic acids in the cane juice, leaves, and bagasse of sugarcane were performed using reversed-phase high-performance liquid chromatography (RP-HPLC). The results showed that phenolic acids primarily existed in the bound form in sugarcane; however, the content and form were different in the three different tissues. The leaves and bagasse had higher phenolic acid content than cane juice. The free phenolic acid content in cane juice, leaves, and bagasse was 317.18 mg/kg (dry solids), 1568.37 mg/kg (dry basis), and 1504.57 mg/kg (dry basis), respectively. The bound phenolic acid content in cane juice, leaves and bagasse was 740.32 mg/kg (dry solids), 2725.41 mg/kg (dry basis), and 2452.13 mg/kg (dry basis). In cane juice, caffeic acid and gallic acid were primarily in the free form, whereas ferulic acid and coumaric acid were primarily in the bound form. Caffeic acid, ferulic acid, and coumaric acid were primarily in the bound form in cane leaves and bagasse. In the different sugarcane tissues, free phenolic acids exhibited better free-radical removal activities towards DPPH and ABTS than bound phenolic acids. The free and bound phenolic acid content in sugarcane tissues was positively correlated with antioxidant activity.
