Hepatoprotective Effect of Burdock Extracts against Carbon Tetrachloride-induced Liver Damage in Mice
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Abstract:
The hepaprotective effects of four burdock extracts on CCl4-induced liver damage in mice, as well as the possible underlying mechanism, were studied. Ethanol and water were used to extract burdock peel and peeled burdock root to yield an ethanolic extract of burdock peel (LP), a water extract of burdock peel (PW), an ethanolic extract of peeled burdock root (MA), and a water extract of peeled burdock root (MW). Biochemical methods were used to measure aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and lactic dehydrogenase (LDH) activities, total bilirubin (TB) level in the serum, as well as malondialdehyde (MDA) level, and superoxide dismutase (SOD) and glutathione (GSH) activities in the liver in mice. The expression levels of SOD1, SOD2, and xanthine oxidase (XOD) in liver tissues were determined by western blot analysis, and the severity of hepatic lesions in mice was observed. In the LP group, the activities of ALT, AST, ALP, LDH, and the TB level in mouse serum were signi?cantly increased (up to >97%). The MDA level in the liver decreased significantly, whereas the GSH and SOD activities increased significantly (up to 45%). In the liver, SOD2 expression increased, but XOD expression decreased. Hepatic lesions were less severe in the LP group than in the CCl4 group. Serum ALT and AST activities were significantly lower in the PW, MA, and MW groups than in the CCl4 group, but no significant differences were observed in other biochemical indicators or in the severity of hepatic lesions. The hepaprotective effect of LP against CCl4-induced liver damage in mice may be achieved by in vivo scavenging of free radicals to maintain the stability of hepatocytes.
